Advancing EV Research: Analytical Chromatography Solutions for Efficient and Comprehensive Characterization of EV Preparations With Varying Complexity
Presented by Darja Božič, Project Manager at Sartorius BIA Separations
The field of extracellular vesicles (EV) has been increasingly empowered in recent decades, with significant advances in sophisticated analytical tools and the development of high-tech applications. However, the isolation and effective analysis of EV samples remain a substantial challenge, posing time and financial burdens that often limit the scope of studies. Comprehensive characterization of EV preparations using several complementary techniques often results in large sample consumption during downstream process development and control, reducing the amount of the final product available for higher-purpose assays.
Is it possible to streamline EV analysis by reducing reliance on multiple techniques and enhancing efficiency in downstream monitoring and quality control? Learn how we reduce the laborious multi-method evaluation of samples at early stages of process development through the efficient extraction of information on particle size, protein content, and specific markers provided by a single chromatography experiment using advanced detection methods such as light absorbance, fluorescence, and light scattering. The aim of the webinar is to highlight the strengths and limitations of Analytical Size Exclusion Chromatography (SEC) in comparison to the conventional orthogonal methods. Key factors to consider when setting up new methods, including ensuring reproducible immunolabeling, will be addressed. We will also introduce a two-dimensional liquid chromatography approach, providing deeper insight into the heterogeneity of biological nanoparticles of similar size.
This webinar is tailored for scientists and professionals eager to overcome the challenges posed by the diverse nature of EVs and the intricate biological matrices they inhabit. It is designed to support experts in setting up their own practical tools to advance EV research and therapeutic applications.
Speaker Bio
Darja Božič is a research scientist and project manager at Sartorius BIA Separations. She studied at University of Ljubljana where she finished bachelor’s in Laboratory Biomedicine from Faculty of Farmacy, and master’s in Biochemistry from Faculty of Chemistry and Chemical Technology. After graduation, she joined the Laboratory of Clinical Biophysics, led by prof. dr. Veronika Kralj-Iglič and obtained her PhD in Biosciences in 2021.
Her research interests include nanostructures, biological communication pathways and analytics. At Sartorius BIA separations, she is currently involved in the development of chromatographic isolation and analysis of extracellular vesicles and their mimetics.
Innovative Contamination Control in Cell Therapy: Enabling Integrity and Efficiency
Presented by George Prout and Suzy Brown
Effective contamination control is essential for maintaining the integrity and quality of cell therapy manufacturing. This presentation will discuss the use of upstream solutions such as automated closed systems for isolation and activation, and larger bioprocess containers. It will also cover the application of analytical assays for sterility testing, mycoplasma detection, and environmental monitoring. These measures aim to reduce contamination and improve overall operational effectiveness. An integrated approach combining these solutions can enhance contamination control, boost process efficiency, and ensure the production of high-quality cell therapy products.
Learning Objectives:
- Learn about the role of automated closed systems and large bioprocess containers in preserving the integrity of cell therapy products.
- Discover the benefits of rapid analytical assays for reliable and accurate contamination detection.
- Consider how an integrated approach can enhance overall process efficiency and ensure high-quality outcomes.
Speaker Bio
George Prout – Field Application Scientist
George Prout is an Application Scientist at Thermo Fisher Scientific covering Cell Culture and Cell Therapy. His role involves technical support with customers wanting to drive higher output of their systems and actively looks for collaborations with fellow scientists in disciplines such as protein production, plasmid production, vaccines, cultured meat, microbial processes, gene therapy and cell therapy from research, to process development and scale-up. George has 8 years of experience in Gene Therapy prior to joining Thermo Fisher Scientific, with a focus on manufacturing, process development and project management.
Suzy Brown - Senior Field Application Scientist
Suzy Brown is the Senior Field Application Scientist for Pharma Analytics, supporting customers in the UK and Ireland. She has been with Thermo Fisher Scientific for 8 years where she provides training and implementation of the Pharma Analytics portfolio. During this time, she has taken on the role of MycoSEQ subject matter expert for the EMEA region.
Suzy gained a Cell Biology Degree, and later a Developmental Biology PhD at the University of Manchester (UK) where she applied molecular, proteomic and Next Generation Sequencing approaches to identify genes required for pattern formation in model organism Dictyostelium discoideum development. Following university, Suzy moved into the Biopharmaceutical Industry and worked as an Analytical Development Scientist for 3 years at Allergan Biologics, within a Molecular Biology team and has experience in qPCR, Sanger Sequencing and impurity testing.